Transparent Electrochemical Detectors to Study Exocytosis
نویسندگان
چکیده
Neurons release neurotransmitters in a process called exocytosis, wherein packets of transmitter molecules are released from the cells upon stimulation. Adrenal chromaffin cells, which also undergo exocytosis, constitute a model system for neuronal release. Quantal release of adrenaline from chromaffin cells can be measured using the electrochemical technique of amperometry, historically employing a carbon fiber electrode [1]. To gain information about the exocytotic mechanism, we have developed a method of electrochemical imaging employing transparent microfabricated devices based on amperometry. We have successfully measured electrochemical events using transparent electrodes simultaneously with fluorescence imaging, yielding more information about the exocytotic process.
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